In-line whole blood fractionation for Raman analysis of blood plasma

Blood analysis by Raman spectroscopy.

Concentrations of multiple analytes were simultaneously measured in whole blood with clinical accuracy, without sample processing, using near-infrared Raman spectroscopy. Spectra were acquired with an instrument employing nonimaging optics, designed using Monte Carlo simulations of the influence of light-scattering-absorbing blood cells on the excitation and emission of Raman light in turbid me...

Stereospecific Determination of Mefloquine in Whole Blood by HPLC

Mefloquine, as a racemic mixture, is used for the treatment and prophylaxis of malaria. Stereoselective pharmacodynamic and pharmacokinetic differences has been observed for mefloquine. In this study a modified stereoselective HPLC method is presented for determination of mefloquine (MFQ) enantiomers in whole blood. The assay involved liquid-liquid extraction of MFQ from biological fluids with ...

Instrumental bias in blood gas analysis of tonometered whole blood.

My purpose in investigating the effect of hemolysis on the accurate assessment of catecholamines and DOPA concentrations by the “Cat-A-Kit” radioenzymic method was to provide a basis for rejecting hemolyzed specimens, and thereby reduce the ever-growing backlog of specimens awaiting analysis in my freezer. I was surprised and disappointed to find that I could not discard hemolyzed samples as be...

Microfluidic Devices for Blood Fractionation

Blood, a complex biological fluid, comprises 45% cellular components suspended in protein rich plasma. These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research. Yet, processing blood is not trivial. In the past deca...

Plasma Fractionation in Malaysia